EB1 Directly Regulates APC-Mediated Actin Nucleation

Current Biology. 2020. [recently accepted]

Maria Angeles Juanes, Colby Fees, Gregory J. Hoeprich, Richa Jaiswal, and Bruce L. Goode

Cell-substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime

PLoS Biol. 2020 Aug 3;18(8):e3000774. doi: 10.1371/journal.pbio.3000774. Online ahead of print. 

Singh SP, Thomason PA, Lilla S, Schaks M, Tang Q, Goode BL, Machesky LM, Rottner K, Insall RH.

Cofilin loss in Drosophila muscles contributes to muscle weakness through defective sarcomerogenesis during muscle growth

Cell Reports. Vol 32, Issue 3, 21 July 2020

Mridula Balakrishnan, Shannon F. Yu, Samantha M. Chin, David B. Soffar, Stefanie 
E. Windner, Bruce L. Goode, and Mary K. Baylies.

WAVE 1 and WAVE 2 have distinct and overlapping roles in controlling actin assembly at the leading edge

MBoC. 2020 Sep 15;31(20):2168-2178. doi: 10.1091/mbc.E19-12-0705. Epub 2020 Jul 22.

Qing Tang, Matthias Schaks, Neha Koundinya, Changsong Yang, Luther W. Pollard, Tatyana M. Svitkina, Klemens Rottner, Bruce L. Goode.

A septin-Hof1 scaffold at the yeast bud neck binds and organizes actin cables

Mol Biol Cell. 2020 Aug 15;31(18):1988-2001. doi: 10.1091/mbc.E19-12-0693. Epub 2020 Jun 24.

Garabedian MV, Wirshing A, Vakhrusheva A, Turegun B, Sokolova OS, Goode BL. A 

Centering and symmetry breaking in confined contracting actomyosin networks

eLife 2020 Apr 21;9. doi: 10.7554/eLife.55368.

Ierushalmi N, Malik-Garbi M, Manhart A, Abu Shah E, Goode BL, Mogilner A, Keren K

Genetically-inspired in vitro reconstitution of S. cerevisiae actin cables from seven purified proteins

Molecular Biology of the Cell 2020. Mar 1;31(5):335-347. doi: 10.1091/mbc.E19-10-0576

Pollard LWGarabedian MVAlioto SLShekhar SGoode BL.



Synergy between Cyclase-associated protein and Cofilin accelerates actin filament depolymerization by two orders of magnitude

The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover

Journal of Cell Biology

Marie Angeles Juanes, Daniel Isnardon, Ali Badache, Sophie Brasselet, Manos Mavrakis, and Bruce L. Goode

Tropomyosin Isoforms differentially tune actin filament length and disassembly

Mol. Biol. Cell. Vol. 30, No. 5.

Silvia Jansen, and Bruce L. Goode

Scaling behavior in steady-state contracting actomyosin networks

Nature Physics 15, pages 509–516.

Maya Malik-Garbi, Niv Ierushalmi, Silvia Jansen, Enas Abu-Shah, Bruce L. Goode, Alex Mogilner and Kinneret Keren



A novel mode of Capping Protein-regulation by Twinfilin

eLife 2018;7:e41313

Adam B. Johnston, Denise M. Hilton, Patrick McConnell, Britney Johnson, Meghan T. Harris, Avital Simone, Gaya K. Amarasinghe, John A. Cooper, and Bruce L. Goode

Integrated control of formin-mediated actin assembly by a stationary inhibitor and a mobile activator

Journal of Cell Biology Oct 1;217(10):3512-3530

Mikael V. Garabedian, Tatiana Stanishneva-Konovalova, Chenyu Lou, Thomas J. Rands, Luther W. Pollard, Olga S. Sokolova and Bruce L. Goode

Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF

Nature Communications Jul 24;9(1):2895

Siyang Guo, Olga S. Sokolova, Johnson Chung, Shae Padrick, Jeff Gelles, and Bruce L. Goode

Species-specific Functions of Twinfilin in Actin Filament Depolymerization

Journal of Molecular Biology Sep 14;430(18 Pt B):3323-3336

Hilton DM, Aguilar RM, Johnston AB, and Goode BL

GMF as an Actin Network Remodeling Factor

Trends Cell Biol. May 18. pii: S0962-8924(18)30072-2.

Goode BL, Sweeney MO, Eskin JA

Structural basis of actin monomer re-charging by cyclase-associated protein

Nature Communications May 14;9(1):1892. doi: 10.1038/s41467-018-04231-7

Tommi Kotila, Konstantin Kogan, Giray Enkavi, Siyang Guo, Ilpo Vattulainen, Bruce L. Goode & Pekka Lappalainen

Rapid Production of Pure Recombinant Actin Isoforms in Pichia

Journal of Cell Science : jcs.213827 doi: 10.1242/jcs.213827

Tomoyuki Hatano, Salvatore Alioto, Emanuele Roscioli, Saravanan Palani, Scott T Clarke, Anton Kamnev, Juan Ramon Hernandez-Fernaud, Lavanya Sivashanmugam, Bernardo Chapa-Y-Lazo, Alexandra Jones, Robert Robinson, Karuna Sampath, Masanori Mishima, Andrew D McAinsh, Bruce Goode, and Mohan Balasubramaniam

A Flow Cytometry-Based Phenotypic Screen To Identify Novel Endocytic Factors in Saccharomyces cerevisiae

G3: Genes, Genomes, Genetics. 2018 Mar 14. pii: g3.200102.2018. doi: 10.1534/g3.118.200102.

Kristie Wrasman, Salvatore Alioto, Yorke Zhang, Kyle Hoban, Marjon Khairy, Bruce Goode, Beverly Wendland



Profilin Directly Promotes Microtubule Growth Through Residues Mutated in Amyotrophic Lateral Sclerosis

Current Biology, 2017  Nov 1. pii: S0960-9822(17)31305-2.

Jessica L. Henty-Ridilla, M. Angeles Juanes, and Bruce L. Goode

APC nucleates actin assembly to drive cell migration and microtubule-induced focal adhesion turnover

Journal of Cell Biology, 2017 Sep 4;216(9):2859-2875.

M. Angeles Juanes, Habib Bouguenina, Julian A. Eskin, Richa Jaiswal, Ali Badache and Bruce L. Goode

Structural basis of Arp2/3 complex inhibition by GMF, Coronin, and Arpin

Journal of Molecular Biology, 2017 Jan 20;429(2):237-248.

Sokolova OS, Chemeris A, Guo S, Alioto S, Gandhi M, Padrick S, Pechnikova E, David V, Gautreau A, and Goode BL.



Tropomyosin and profilin cooperate to promote formin-mediated actin nucleation and drive yeast actin cable assembly

Current Biology, 2016 Nov 15. pii: S0960-9822(16)31138-1.
Salvatore L. Alioto, Mikael V. Garabadian, Danielle Bellavance, and Bruce L. Goode ​

Accelerated actin filament polymerization from microtubule plus ends

Science. 2016 May 20;352(6288):1004-9.

Henty-Ridilla JL, Rankova A, Eskin JA, Kenny K, Goode BL.

TIRF microscopy analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover

J. Mol. Biol. 2016 Mar 17. pii: S0022-2836(16)00192-3.
Chin, S.M., Jansen, S., and Goode, B.L.

Design Principles of Length Control of Cytoskeletal Structures

Ann. Rev. Biophys. 2016 June.
Mohapatra, L., Goode, B.L., Jelenkovic, P., Phillips, R., and Kondev, J.

Common formin-regulating sequences in Smy1 and Bud14 are required for the control of actin cable assembly in vivo

Mol. Biol. Cell. 2016 Mar 1; 27(5):828-37.
Eskin, J.A., Rankova, A., Johnston, A.B., Alioto, S.L., and Goode, B.L.



High Speed Depolymerization at Actin Filament Ends Jointly Catalyzed by Twinfilin and Srv2/CAP

Nat. Cell Biol. 2015 Sep 11; 17(11): 1504-1511.
Johnston, A.J., Collins, A., and Goode, B.L.

Single-molecule visualization of a formin-capping protein 'decision complex' at the actin filament barbed-end

Nat. Comm. 2015 Sep 11; 6: 8707-8024.
Bombardier, J., Eskin, J.A., Jaiswal, R., Correa, I.R., Xu, M-Q, Goode, B.L., and Gelles, J.

Coronin enhances actin filament severing by recruiting cofilin to filament sides and altering F-actin conformation

J. Mol. Biol. 2015 Aug 15; 427(19): 3137-3147.
Mikati, M.A.*, Breitsprecher, D.*, Jansen, S.*, Reisler, E., and Goode, B.L

Combinatorial genetic analysis of a network of actin disassembly-promoting factors

Cytoskeleton. 2015 Jul 3; 72(7): 349-361.
Ydenberg, C.A., Johnston, A., Weinstein, J., Bellavance, D., Jansen, S., and Goode, B.L.

Structure of a Bud6/actin complex reveas a novel WH2-like actin monomer recruitment motif

Structure. 2015 May 15; 23: 1-8.
Park, E., Graziano, B.R., Zheng W., Garabedian, M., Goode, B.L., and Eck, M.J.

Structural basis for mutation-induced destabilization of Profilin 1 in ALS

Proc. Nat. Acad. Sci. 2015 Jun 30; 112(26):7984-9.
Boopathy, S., Silvas, T., Tischbein, M., Jansen, S., Shandilya, S.M.D., Zitzewitz, J.A., Landers, J.E., Goode, B.L., Schiffer, C.A., and Bosco, D.A.

Single molecule imaging of a three-component ordered actin disassembly mechanism

Nat. Comm. 2015 April 17; (6):7202.
Jansen, S., Collins, A., Chin, S.M., Ydenberg, C.A., Gelles, J. and Goode, B.L.



A novel role for WAVE1 in controlling actin network growth rate and architecture

Mol. Biol. Cell. 2014 December 3. E14-10-1477
Sweeney, M.O., Collins, A., Padrick, S.B., and Goode, B.L.

Structure and mechanism of mouse cyclase-associated protein (CAP1) in regulating actin dynamics

J. Biol. Chem. 2014 Oct 31; 289(44):30732-42.
Jansen, S., Collins, A., Golden, L., Sokolova, O., and Goode, B.L.

GMF promotes leading edge dynamics and collective cell migration in vivo

Curr. Biol. 2014 Nov 3; 24(21):2533-40.
Poukkula, M., Hakala, M., Pentinmikko, N., Sweeney, M., Jansen, S., Mattila, J., Hietakangas, V., Goode, B.L., and Lappalainen, P.

S. cerevisiae Kelch proteins and Bud14 form a stable 520 kDa formin-regulatory complex that controls actin cable assembly and cell morphogenesis

J. Biol. Chem. 2014 June 27; 289(26):18290-301.
Gould, C.J., Chesarone-Cataldo, M., Alioto, S.L., Salin, B., Sagot, I., and Goode B.L.

The F-BAR protein Hof1 tunes formin activity to sculpt actin cables during polarized growth

Mol. Biol. Cell. 2014 June 5; 25(11):1730-43.
Graziano, B.R., Yu, H-Y., Alioto, S.L., Eskin, J.A., Ydenberg, C.A., Waterman, D.P., Garabedian, M., and Goode B.L.

Single-molecule studies of actin assembly and disassembly factors

Meth Enz. 2014 Mar 10; 540:95-117. 
Smith, B., Gelles J., and Goode B.L.

Autonomous and in trans functions for the two halves of Srv2/CAP in promoting actin turnover

Cytoskeleton. 2014 June; 71(6):351-60.
Chaudhry, F., Jansen, S., Little, K., Suarez, C., Boujemaa-Paterski, R., Blanchoin, L., and Goode B.L.

Critical roles for multiple formins during cardiac myofibril development and repair

Mol Biol Cell. 2014 Jan 15; doi: 10.1091/mbc.E13-08-0443.
Rosado, M., Barber, C.F., Berciu, C., Feldman, S., Birren, S.J., Nicastro, D., Goode, B.L.

Essential and non-redundant roles for Diaphanous formins in cortical microtubule capture and directed cell migration

Mol Biol Cell. 2014 Mar; 25(5):658-68.
Daou, P., Hasan, S., Breitsprecher, D., Baudelet, E., Camoin, L., Audebert, S., Goode, B.L., Badache, A.



Three-color single-molecule imaging shows WASP release from the nascent branch triggers actin nucleation by Arp2/3 complex

eLife. 2013 Sep 3; 2:e01008. 
Smith, B.A., Padrick, S.B., Doolittle, L.K., Daugherty-Clarke, K., Corrêa Jr., I.R., Xu, M-Q., Goode, B.L., Rosen, M.K., Gelles, J.

The Formin Daam1 and Fascin Directly Collaborate to Promote Filopodia Formation

Curr. Biol. 2013 Jul 22; 23(14):1373-9.
Jaiswal, R., Breitsprecher, D., Collins, A.,Corrêa Jr., I.R., Xu, M-Q., and Goode, B.L.

GMF severs actin-Arp2/3 complex branch junctions by a cofilin-like mechanism

Curr. Biol. 2013 June 7; 23(12):1037-45.
Ydenberg, C.A., Padrick, S.B., Sweeney, M.O., Gandhi, M., Sokolova, O., and Goode, B.L.

Drosophila homologues of Adenomatous Polyposis Coli (APC) and the formin Diaphanous collaborate by a conserved mechanism to stimulate actin filament assembly

J. Biol. Chem. 2013 May 10; 288(19):13897-905.
Jaiswal, R., Stepanik, V., Rankova, A., Molinar, O., Goode, B.L. and McCartney, B.M.

Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation

J Cell Biol. 2013 May 13; 201(4):595-611.
Graziano, B.R., Jonasson, E.M., Pullen, J.G., Gould, C.J., and Goode, B.L.

Formins at a glance

J Cell Sci. 2013 Jan 1; 126(Pt 1):1-7. 
Breitsprecher, D., and Goode, B.L.

Pathway of actin filament branch formation by Arp2/3 complex revealed by single molecule imaging

PNAS. Jan 4, 2013; doi:10.1073/pnas.1211164110
Smith B.A., Daugherty-Clarke, K., Goode, B.L, and Gelles, J.

Srv2/cyclase-associated protein forms hexameric shurikens that directly catalyze actin filament severing by cofilin

Mol Biol Cell. 2013 Jan; 24(1):31-41. doi:10.1091/mbc.E12-08-0589.

Chaudhry F, Breitsprecher D, Little K, Sharov G, Sokolova O and Goode BL.



Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6

Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):E3424-33. doi: 10.1073/pnas.1203035109.
Tu D, Graziano BR, Park E, Zheng W, Li Y, Goode BL, Eck MJ.

Structure and activity of full-length formin mDia1

Cytoskeleton (Hoboken). 2012 Jun;69(6):393-405. doi: 10.1002/cm.21033.
Maiti S, Michelot A, Gould C, Blanchoin L, Sokolova O, Goode BL.

Rocket launcher mechanism of collaborative actin assembly defined by single-molecule imaging

Science. 2012 Jun 1;336(6085):1164-8. doi: 10.1126/science.1218062.
Breitsprecher D, Jaiswal R, Bombardier JP, Gould CJ, Gelles J, Goode BL.



Cease-fire at the leading edge: new perspectives on actin filament branching, debranching, and cross-linking

Cytoskeleton (Hoboken). 2011 Nov;68(11):596-602. doi: 10.1002/cm.20543.

Ydenberg CA, Smith BA, Breitsprecher D, Gelles J, Goode BL.

Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor

Mol Biol Cell. 2011 Nov;22(21):4016-28. 

Graziano BR, DuPage AG, Michelot A, Breitsprecher D, Moseley JB, Sagot I, Blanchoin L, Goode BL.

Cofilin cooperates with fascin to disassemble filopodial actin filaments

J Cell Sci. 2011 Oct 1;124(Pt 19):3305-18. doi: 10.1242/jcs.086934.

Breitsprecher D, Koestler SA, Chizhov I, Nemethova M, Mueller J, Goode BL, Small JV, Rottner K, Faix J.

The myosin passenger protein Smy1 controls actin cable structure and dynamics by acting as a formin damper

Dev Cell. 2011 Aug 16;21(2):217-30. doi: 10.1016/j.devcel.2011.07.004.

Chesarone-Cataldo M, Guérin C, Yu JH, Wedlich-Soldner R, Blanchoin L, Goode BL.

Crystal structure of a coiled-coil domain from human ROCK I

PLoS One. 2011 Mar 21;6(3):e18080. doi: 10.1371/journal.pone.0018080.

Tu D, Li Y, Song HK, Toms AV, Gould CJ, Ficarro SB, Marto JA, Goode BL, Eck MJ.

The formin DAD domain plays dual roles in autoinhibition and actin nucleation

Curr Biol. 2011 Mar 8;21(5):384-90. doi: 10.1016/j.cub.2011.01.047.

Gould CJ, Maiti S, Michelot A, Graziano BR, Blanchoin L, Goode BL.

Coronin 2A mediates actin-dependent de-repression of inflammatory response genes

Nature. 2011 Feb 17;470(7334):414-8. doi: 10.1038/nature09703.

Huang W, Ghisletti S, Saijo K, Gandhi M, Aouadi M, Tesz GJ, Zhang DX, Yao J, Czech MP, Goode BL, Rosenfeld MG, Glass CK.



Functional surfaces on the actin-binding protein coronin revealed by systematic mutagenesis

J Biol Chem. 2010 Nov 5;285(45):34899-908. doi: 10.1074/jbc.M110.171496.

Gandhi M, Jangi M, Goode BL

Adenomatous polyposis coli protein nucleates actin assembly and synergizes with the formin mDia1

J Cell Biol. 2010 Jun 28;189(7):1087-96. doi: 10.1083/jcb.201001016.

Okada K, Bartolini F, Deaconescu AM, Moseley JB, Dogic Z, Grigorieff N, Gundersen GG, Goode BL.

GMF is a cofilin homolog that binds Arp2/3 complex to stimulate filament debranching and inhibit actin nucleation

Curr Biol. 2010 May 11;20(9):861-7. doi: 10.1016/j.cub.2010.03.026.

Gandhi M, Smith BA, Bovellan M, Paavilainen V, Daugherty-Clarke K, Gelles J, Lappalainen P, Goode BL.

A central role for the WH2 domain of Srv2/CAP in recharging actin monomers to drive actin turnover in vitro and in vivo

Cytoskeleton (Hoboken). 2010 Feb;67(2):120-33. doi: 10.1002/cm.20429.

Chaudhry F, Little K, Talarico L, Quintero-Monzon O, Goode BL.